Human Lamin B2

U2os human osteosarcoma cell line whole cell lysate lane 4.

Human lamin b2.

80 9 80 92 mb pubmed search wikidata view edit human view edit mouse lamin b2 is a protein that in humans is encoded by the lmnb2 gene. Mutations in this gene are associated with acquired partial lipodystrophy. It remains to be determined to what degree increasing lamin b2 protein expression levels would alter heart regeneration in adult mice. The presented results in mouse human and zebrafish suggest that one characteristic of a proliferative cardiomyocyte subpopulation would be higher expression levels of lamin b2 protein.

The increased labelling observed with the anti lamin b2 b3 antibody compared with the anti lamin b2 antibody taken together with the extremely low level of lamin b2 transcripts in the spermatozoa of at least four of the five men tested here is in favour of the presence of the lamin b3 protein in human spermatids but a specific antibody will be required to confirm this. Control with 3 µg of pre immune rabbit igg c. 16907 ensembl ensg00000176619 ensmusg00000062075 uniprot q03252 p21619 refseq mrna nm 032737 nm 010722 nm 001347140 refseq protein np 116126 np 001334069 np 034852 location ucsc chr 19. However lmnb2 contains an intron between the regions encoding coil 1a and coil 1b that is not present in other if proteins including lmna indicating that lmnb2 and lmna evolved from an lmnb1 like ancestor.

Hela human epithelial carcinoma cell line whole cell lysate lane 2. Jurkat human t cell lymphoblast like cell line whole cell lysate lane 3. Immunoprecipitation of lamin b2 by 3 µg of lamin b2 antibody n3c2 internal 107 10877 7 5 sds page the immunoprecipitated. Lamin b2 epididymis secretory sperm binding protein lamin b3.

The lamin family of proteins make up the matrix and are highly conserved in evolution. View protein in pfam pf00038 filament 1 hit pf00932 ltd 1 hit. The nuclear lamina consists of a two dimensional matrix of proteins located next to the inner nuclear membrane. 2 43 2 46 mb chr 10.

Anti lamin b2 antibody ln43 ab8983 at 1 µg ml lane 1. Lamin proteins are thought to be involved in nuclear stability chromatin structure and gene expression. Vertebrate lamins consist of two types a and b. Rna primed nascent dna molecules were subjected to second strand synthesis with appropriate primers amplified by ligation mediated polymerase chain reaction and size fractionated.

Hepg2 whole cell lysate extract a. Lamin b2 antibody n3c2 internal immunoprecipitates lamin b2 protein in ip experiments. The initiation sites of bidirectional synthesis at the dna replication origin located at the 3 end of the human lamin b2 gene were investigated. For example intron positions of the human lamin b1 gene lmnb1 are conserved in the lamin a c lmna and lamin b2 lmnb2 genes.

Hl60 human promyelocytic leukemia cell line whole cell lysate lysates proteins at 10 µg per lane. Provided by refseq may 2012 other designations. 30 µg whole cell lysate extract of lamin b2 protein expressing hepg2 cells b. Evidence for precise start sites was obtained.